Journal: Journal of Clinical Medicine

Article Title: Regulatory T Cells Modulate CD4 Proliferation after Severe Trauma via IL-10

doi: 10.3390/jcm9041052

Figure Lengend Snippet: Gating strategy for the flow cytometric analysis. ( A ): Gating of the lymphocyte fraction in peripheral blood according to the forward and side scatter. ( B ): Gating for the detection of CD4 + CD25 + cells. ( C ): Gating of the lymphocytes according to their CD4 and CD25 expression and subsequently of CD127 and FoxP3 expressing cells.

Article Snippet: The samples were incubated for 30 min at room temperature in the dark with mouse anti-human CD4 Brilliant Violett (Clone RPA-T4, BD Bioscience, San Diego, CA, USA), mouse anti-human CD127 PerCP-Cy5.5 (Clone HIL-7R-M21, BD Bioscience, San Diego, CA, USA), mouse anti-human CD25 APC-Cy7 (Clone BC 96, BD Bioscience, San Diego, CA, USA), and mouse anti-human FoxP3 PE-Cy7 (Clone PCH101, BD Bioscience, San Diego, CA, USA) antibodies.

Techniques: Expressing

Journal: Journal of Clinical Medicine

Article Title: Regulatory T Cells Modulate CD4 Proliferation after Severe Trauma via IL-10

doi: 10.3390/jcm9041052

Figure Lengend Snippet: Whole blood from healthy volunteers (ctrl, n = 10, clear symbols) or major trauma patients (TP, n = 29, black symbols) was analyzed by flow cytometry over a 10-day time course after admission (emergency department, ED-10). Regulatory T cells (Tregs) were detected using anti-human CD4 and CD25 in the corresponding side and forward scatter. Unstimulated (square) and stimulated (circle, stim) cells were measured. Data are shown as mean ± SEM percentage of lymphocytes. ( A ): CD4 + CD25 +/low Tregs to CD4 + lymphocytes; ( B ): CD4 + CD25 +/high Tregs to CD4 + lymphocytes; ( C ): CD4 + CD25 + CD127 − Tregs to CD4 + lymphocytes; ( D ): CD4 + CD25 + FoxP3 + Tregs to CD4 + lymphocytes. p < 0.05 * vs. indicated #; unstimulated TP vs. unstimulated crtl $; stimulated TP vs. unstimulated TP.

Article Snippet: The samples were incubated for 30 min at room temperature in the dark with mouse anti-human CD4 Brilliant Violett (Clone RPA-T4, BD Bioscience, San Diego, CA, USA), mouse anti-human CD127 PerCP-Cy5.5 (Clone HIL-7R-M21, BD Bioscience, San Diego, CA, USA), mouse anti-human CD25 APC-Cy7 (Clone BC 96, BD Bioscience, San Diego, CA, USA), and mouse anti-human FoxP3 PE-Cy7 (Clone PCH101, BD Bioscience, San Diego, CA, USA) antibodies.

Techniques: Flow Cytometry

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: Immune cell infiltration in KRAS G12C mutant PNETs (A) Quantitative analysis using Image Plus 6.0 software reveals a significant increase in Tregs (CD4 + , CD25 + , and FoxP3 + ) within KRAS- G12C mutant PNETs compared to wild-type KRAS PNET tissues, suggesting KRAS G12C -driven immunosuppressive cell recruitment. (B) Fluorescence intensity analysis demonstrates reduced infiltration of CD8 + cytotoxic T cells and HLA-DR + activated cells in KRAS G12C mutant tumors, indicative of impaired antitumor immunity. (C) Elevated MDSCs counts in KRAS G12C mutant PNETs correlate with enhanced immune evasion.

Article Snippet: PE Anti-Human CD25 Antibody [BC96] , Elabscience , E-AB-F1194D.

Techniques: Mutagenesis, Software, Fluorescence

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: Tregs, CD8 + T cells, and HLA-DR + cells in KRAS G12C -mutated PNETs (A) Flow cytometry plots and fluorescence intensity histograms demonstrate elevated CD4 + T cell proportions in KRAS G12C patient blood samples compared to wild-type KRAS tumors and healthy controls. (B) Quantification shows a significant enrichment of CD25 + T cells in KRAS G12C patients, surpassing both wild-type KRAS tumors and normal controls. (C) Quantitative data and histogram overlays confirm a substantial increase in FoxP3 + T cells frequency in KRAS G12C patients, with levels moderately elevated compared to wild-type KRAS and significantly higher than healthy individuals. (D) A slight decrease in CD8 + T cell frequency in KRAS G12C samples relative to wild-type KRAS, with levels significantly lower than those in healthy individuals (E) A moderate reduction in HLA-DR + cell frequency in KRAS G12C patients compared to wild-type KRAS, alongside a marked suppression relative to healthy controls.

Article Snippet: PE Anti-Human CD25 Antibody [BC96] , Elabscience , E-AB-F1194D.

Techniques: Flow Cytometry, Fluorescence